KDELR2 antibody

Catalog No. ABIN498960

Product Details anti-KDELR2 Antibody

Target: KDELR2 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 2 (KDELR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDELR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of KDEL Receptor 2 protein. (region surrounding Leu119)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for KDELR2

Target: KDELR2 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 2 (KDELR2))

Alternative Name: KDEL Receptor 2 / KDELR2 (KDELR2 Products)

Synonyms: kdelr2 antibody, ELP-1 antibody, ERD2.2 antibody, 1110007A14Rik antibody, kdelr1-a antibody, KDEL endoplasmic reticulum protein retention receptor 2 L homeolog antibody, KDEL endoplasmic reticulum protein retention receptor 2 antibody, KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 2 antibody, KDEL endoplasmic reticulum protein retention receptor 1 L homeolog antibody, kdelr2.L antibody, KDELR2 antibody, Kdelr2 antibody, kdelr1.L antibody

Background: Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-gluleu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.Synonyms: ELP-1, ER lumen protein retaining receptor 2, ERD2-like protein 1, ERD2.2, KDEL endoplasmic reticulum protein retention receptor 2

Molecular Weight: approx. 24 kDa

Gene ID: 11014

NCBI Accession: NP_006845

UniProt: P33947

Pathways: Maintenance of Protein Location