NFKBIB antibody

Catalog No. ABIN498987

The Rabbit Polyclonal anti-NFKBIB antibody (ABIN498987) specifically detects NFKBIB in WB. The antibody is reactive with Human samples.

Quick Overview for NFKBIB antibody (ABIN498987)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFKBIB Antibody

Specificity: This antibody detects endogenous levels of IkappaB-beta protein. (region surrounding Ala13)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: NFKBIB / IKBB

Background: Three major forms of IkappaB-like molecules have been identified and each is characterized by multiple copies of ankyrin repeats. IkappaB-alpha and IkappaB-beta appear to be the major regulatory forms of IkappaB in most cells. These proteins interact with p65 or c-Rel containing forms of NFkappaB and block nuclear import by masking the nuclear localization sequences of NFkappaB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IkappaB. Immunoblotting easily detects the hyper-phosphorylated forms of IkappaB-alpha, but not phosphorylated IkappaB-beta.Synonyms: I-kappa-B-beta, IkB-B, IkB-beta, IkappaBbeta, NF-kappa-B inhibitor beta, TR-interacting protein 9, TRIP9, Thyroid receptor-interacting protein 9

Molecular Weight: approx. 37 kDa

Gene ID: 4793

NCBI Accession: NP_001001716

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades