STAT4 antibody (Internal Region)

Catalog No. ABIN499124

The Goat Polyclonal anti-STAT4 antibody is suitable to detect STAT4 in samples from Human, Pig and Dog. It has been validated for WB and EIA.

Quick Overview for STAT4 antibody (Internal Region) (ABIN499124)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This STAT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-STAT4 Antibody

Binding Specificity: AA 613-627, Internal Region

Sequence: C-DHSESGEVRF HSVEP

Specificity: Recognizes STAT4 (aa 613-627).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (aa 613-627) of the protein sequence according to Human STAT4 (NP_003142.1.) Genename: STAT4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 85 kDa in transfectedHEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: STAT4

Background: The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4

Gene ID: 6775

NCBI Accession: NP_003142

UniProt: Q14765

Pathways: JAK-STAT Signaling