GLUT4 antibody (C-Term)

Catalog No. ABIN499128

The Goat Polyclonal anti-GLUT4 antibody (ABIN499128) specifically detects GLUT4 in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Pig and Cow samples.

Quick Overview for GLUT4 antibody (C-Term) (ABIN499128)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reactivity: Human, Mouse, Rat, Pig, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This GLUT4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GLUT4 Antibody

Binding Specificity: C-Term

Sequence: C-TELEYLGPDE ND

Specificity: Recognizes SLC2A4 / GLUT4.

Cross-Reactivity (Details): Species reactivity (expected):Human, Cow, Pig.
Species reactivity (tested):Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C-Terminus of the protein of Human SLC2A4 (NP_001033.1). Genename: SLC2A4

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 45 kDa in Mouse and RatHeart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Alternative Name: GLUT4 / SLC2A4

Background: The stimulation of glucose uptake by insulin in muscle and adipose tissue requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.Synonyms: GLUT-4, Glucose transporter 4, Glucose transporter type 4 insulin-responsive, Solute carrier family 2 facilitated glucose transporter member 4

Gene ID: 6517

NCBI Accession: NP_001033

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effect