ANAPC4 antibody (N-Term)

Catalog No. ABIN499297

Product Details anti-ANAPC4 Antibody

Target: ANAPC4 (Anaphase Promoting Complex Subunit 4 (ANAPC4))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Purification: Affinity chromatography purified via peptide column

Immunogen: APC4 antibody was raised against a 14 amino acid peptide near the amino terminus of human APC4.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: APC4 antibody can be used for detection of APC4 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for ANAPC4

Target: ANAPC4 (Anaphase Promoting Complex Subunit 4 (ANAPC4))

Alternative Name: APC4 / ANAPC4 (ANAPC4 Products)

Background: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.Synonyms: Anaphase-promoting complex subunit 4, Cyclosome subunit 4

Gene ID: 29945

UniProt: Q9UJX5

Pathways: M Phase