Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

ASK1 antibody (C-Term)

MAP3K5 Reactivity: Human WB, IF, EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN499345
  • Target See all ASK1 (MAP3K5) Antibodies
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Binding Specificity
    • 26
    • 26
    • 18
    • 17
    • 16
    • 12
    • 11
    • 9
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 182
    • 100
    • 57
    • 18
    • 15
    • 8
    Human
    Host
    • 191
    • 8
    Rabbit
    Clonality
    • 171
    • 28
    Polyclonal
    Conjugate
    • 81
    • 12
    • 12
    • 9
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 1
    This ASK1 antibody is un-conjugated
    Application
    • 158
    • 58
    • 39
    • 39
    • 38
    • 30
    • 29
    • 19
    • 11
    • 11
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
    Specificity
    ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    Human ASK1 Peptide
    Isotype
    IgG
    Top Product
    Discover our top product MAP3K5 Primary Antibody
  • Application Notes
    ELISA. Western Blot: ASK1 antibody can be used for detection of ASK1 at 1: 500 to 1: 1000 dilution. A 155 kDa band can be detected. Immunocytochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
    Alternative Name
    MEKK5 / ASK1 (MAP3K5 Products)
    Synonyms
    ASK1 antibody, MAPKKK5 antibody, MEKK5 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MUD21.11 antibody, MUD21_11 antibody, 7420452D20Rik antibody, ASK antibody, Mekk5 antibody, Ask1 antibody, RGD1306565 antibody, mitogen-activated protein kinase kinase kinase 5 antibody, MAP3K5 antibody, MAPKKK5 antibody, Map3k5 antibody, map3k5 antibody
    Background
    Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
    Gene ID
    4217
    UniProt
    Q99683
    Pathways
    MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
You are here:
Support