Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (C-Term) antibody

Details for Product No. ABIN499345
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Antigen
Synonyms ASK1, MEKK5, MAPKKK5, 7420452D20Rik, ASK, Mekk5, MAP3K5, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11
Epitope
C-Term
(49), (39), (20), (16), (12), (12), (12), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(290), (130), (76), (36), (24), (13)
Host
Rabbit
(273), (20)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(211), (112), (84), (68), (60), (32), (27), (7), (5), (5), (3), (3), (2), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN499345
Quantity 0.1 mg
Price
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Immunogen Human ASK1 Peptide (AP30085CP-N)
Isotype IgG
Specificity ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
Purification Affinity chromatography purified via peptide column
Alternative Name MEKK5 / ASK1
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-alpha-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Alternate names: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
NCBI Accession Q99683
UniProt Q99683
Application Notes ELISA. Western Blot: ASK1 antibody can be used for detection of ASK1 at 1: 500 to 1: 1000 dilution. A 155 kDa band can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Wang, Diener, Jannuzzi et al.: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997 (PubMed).

Ichijo, Nishida, Irie et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997 (PubMed).

Tobiume, Inage, Takeda et al.: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997 (PubMed).

Hosts (273), (20)
Reactivities (290), (130), (76), (36), (24), (13)
Applications (211), (112), (84), (68), (60), (32), (27), (7), (5), (5), (3), (3), (2), (1), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (49), (39), (20), (16), (12), (12), (12), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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