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MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5)

Details for Product anti-MAP3K5 Antibody No. ABIN499347, Supplier: Login to see
Antigen
  • 7420452D20Rik
  • ASK
  • ASK1
  • MAPKKK5
  • Mekk5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • MEKK5
  • MAP3K5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Reactivity
Human, Mouse (Murine)
371
137
78
4
1
Host
Rabbit
357
22
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
12
12
11
6
6
6
6
5
5
5
5
5
5
5
5
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
290
147
127
57
56
38
23
10
7
6
6
2
1
1
1
Supplier
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Immunogen Human ASK1 Peptide
Isotype IgG
Specificity ASK1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ASK1.
Purification Affinity chromatography purified via peptide column
Alternative Name MEKK5 / ASK1 (MAP3K5 Antibody Abstract)
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-?-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.Synonyms: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
UniProt Q99683
Pathways MAPK Signaling
Application Notes ELISA. Western Blot: ASK1 antibody can be used for the detection of ASK1 at 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunocytochemistry of ASK1 in A431 cells with AP30086PU-N ASK1 antibody at 2 μg/ml.
Immunofluorescence (IF) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Immunofluorescence of ASK1 in 3T3 cells with AP30086PU-N ASK1 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (ABIN499347) Western blot analysis of ASK1 in SW1353 cell lysate with AP30086PU-N ASK1 antibody at...
Background publications Tobiume, Inage, Takeda et al.: "Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1." in: Biochemical and biophysical research communications, Vol. 239, Issue 3, pp. 905-10, 1997 (PubMed).

Ichijo, Nishida, Irie et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997 (PubMed).

Wang, Diener, Jannuzzi et al.: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997 (PubMed).