Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (N-Term) antibody

Details for Product No. ABIN499349
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Antigen
Synonyms ASK1, MEKK5, MAPKKK5, 7420452D20Rik, ASK, Mekk5, MAP3K5, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11
Epitope
N-Term
(49), (39), (21), (16), (12), (12), (11), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(290), (129), (76), (36), (24), (13)
Host
Rabbit
(273), (20)
Clonality
Polyclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
ELISA, Western Blotting (WB)
(211), (112), (84), (68), (60), (33), (28), (7), (5), (5), (3), (3), (2), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN499349
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
Shipping to United States (Change)
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human ASK1 (N-Terminus) Peptide (AP30087CP-N)
Isotype IgG
Specificity ASK1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human ASK1.
Purification Affinity chromatography purified via peptide column
Alternative Name MEKK5 / ASK1
Background Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-Alpha-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
Alternate names: Apoptosis signal-regulating kinase 1, MAP3K5, MAPK/ERK kinase kinase 5, MAPKKK5, Mitogen-activated protein kinase kinase kinase 5
Gene ID 4217
NCBI Accession BAA12684
UniProt Q99683
Application Notes ELISA. Western Blot: ASK1 antibody can be used for the detection of ASK1 at 1 – 2 μg/ml. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Wang, Diener, Jannuzzi et al.: "Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase." in: The Journal of biological chemistry, Vol. 271, Issue 49, pp. 31607-11, 1997 (PubMed).

Ichijo, Nishida, Irie et al.: "Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways." in: Science (New York, N.Y.), Vol. 275, Issue 5296, pp. 90-4, 1997 (PubMed).

Hosts (273), (20)
Reactivities (290), (129), (76), (36), (24), (13)
Applications (211), (112), (84), (68), (60), (33), (28), (7), (5), (5), (3), (3), (2), (1), (1), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (49), (39), (21), (16), (12), (12), (11), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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