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Details for Product No. ABIN499351

Activating Transcription Factor 6 (ATF6) (C-Term) antibody

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Antigen
Synonyms ATF6A, ESTM49, AA789574, Atf6alpha, 9630036G24, 9130025P16Rik, ATF-6-like, GB16435, ATF6, atf6, Atf6
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN499351
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Human ATF6 (C-Terminus) Peptide (AP30088CP-N)
Isotype IgG
Specificity This antibody was raised against a 16 amino acid peptide from near the carboxy terminus of Human ATF6. ATF6 has two isoforms (ATF6Alpha and ATF6Beta), only ATF6Alpha is recognized by this antibody.
Purification Affinity chromatography purified via peptide column
Alternative Name ATF6 alpha
Background Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR- responsive genes (2-4).
Alternate names: ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Gene ID 22926
NCBI Accession NP_031374
UniProt P18850
Application Notes ELISA. Western Blot: ATF6 antibody can be used for the detection of ATF6 at 0.5 - 1 μg/ml. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Liu, Kaufman: "The unfolded protein response." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1861-2, 2003 (PubMed).

Alternatives for antigen "Activating Transcription Factor 6 (ATF6)", type "Antibodies"
Hosts (69), (15), (7)
Reactivities (88), (51), (27), (13), (12), (6), (1), (1), (1)
Applications (67), (37), (27), (25), (13), (10), (8), (7), (4), (4), (4), (3), (2)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (17), (17), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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