ATF6 antibody (N-Term)

Catalog No. ABIN2451923

There are 3+ publications for this product available. The Mouse Monoclonal anti-ATF6 antibody is suitable to detect ATF6 in samples from Human and Mouse. It has been validated for WB and IP.

Quick Overview for ATF6 antibody (N-Term) (ABIN2451923)

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 37-1

Product Details anti-ATF6 Antibody

Binding Specificity: N-Term

Specificity: Reactive to human and mouse ATF6alpha. However, clone 1-7 antibody is recommended for human cells.

Characteristics: The antibody was produced from hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Purification: Purified

Sterility: Sterile filtered

Immunogen: Recombinant ATF6alpha (amino-terminal fragment of ATF6alpha fused to GST)

Isotype: IgG1 kappa

Application Details

Application Notes: 1. Western blotting
2. Immunoprecipitation (IP) (less efficient than clone1-7)
This antibody does not work for immunofluorescence analyses.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 50 % Glycerol

Preservative: Azide free

Storage: -20 °C

References for anti-ATF6 antibody (ABIN2451923)

Mori: "Divest yourself of a preconceived idea: transcription factor ATF6 is not a soluble protein!" in: Molecular biology of the cell, Vol. 21, Issue 9, pp. 1435-8, (2010) (PubMed).

Yamamoto, Sato, Matsui, Sato, Okada, Yoshida, Harada, Mori: "Transcriptional induction of mammalian ER quality control proteins is mediated by single or combined action of ATF6alpha and XBP1." in: Developmental cell, Vol. 13, Issue 3, pp. 365-76, (2007) (PubMed).

Hai, Liu, Coukos, Green: "Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers." in: Genes & development, Vol. 3, Issue 12B, pp. 2083-90, (1990) (PubMed).

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 alpha

Background: ATF6 (activating transcription factor 6) is an endoplasmic reticulum (ER) membrane-bound transcription factor activated in response to ER stress. When unfolded proteins accumulate in the ER, ATF6 is cleaved by regulated intramembrane proteolysis. The resulting amino-terminal fragment translocates to the nucleus and activates transcription by binding to ER stress-response elements present in the promoter regions of ER stress-inducible genes including those encoding ER chaperones and components of ER-associated degradation. The mammalian ATF6 family consists of two closely related homologs, ATF6alpha and ATF6beta. ATF6alpha but not ATF6beta plays a pivotal role in transcriptional control. The monoclonal antibody was characterized in the laboratory of Professor Kazutoshi Mori of Kyoto University.

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response