ATF6 antibody (AA 437-449)

Catalog No. ABIN1590087

Product Details anti-ATF6 Antibody

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Binding Specificity: AA 437-449

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: ATF6 (aa437-449)

Sequence: NSYRYDHSVS NDK

Cross-Reactivity: Cow, Dog, Human, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75-80 kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6 kDa according to NP_031374.2). Recommended concentration: 0.3-2 μg/mL. Primary incubation 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 (ATF6 Products)

Synonyms: ATF-6-like antibody, GB16435 antibody, ATF6 antibody, Atf6 antibody, ATF6A antibody, 9130025P16Rik antibody, 9630036G24 antibody, AA789574 antibody, Atf6alpha antibody, ESTM49 antibody, activating transcription factor 6 antibody, uncharacterized LOC412432 antibody, activating transcription factor 6 S homeolog antibody, cyclic AMP-dependent transcription factor ATF-6 alpha antibody, ATF6 antibody, LOC412432 antibody, atf6.S antibody, LOC100122162 antibody, Atf6 antibody

Background: ATF6, activating transcription factor 6, ATF6A, cAMP-dependent transcription factor ATF-6 alpha, cyclic AMP-dependent transcription factor ATF-6 alpha

Gene ID: 22926, 304962

NCBI Accession: NP_031374

Pathways: ER-Nucleus Signaling, Unfolded Protein Response