BMI1 antibody (Intermediate Domain)

Catalog No. ABIN499483

This Rabbit Polyclonal antibody specifically detects BMI1 in WB, IF and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BMI1 antibody (Intermediate Domain) (ABIN499483)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-BMI1 Antibody

Binding Specificity: Intermediate Domain

Specificity: BMI-1 antibody was raised against a peptide corresponding to 15 amino acids near the center of human BMI-1.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human BMI-1 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: BMI-1 antibody can be used for detection of BMI-1 at 0.5 to 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI-1 / RNF51

Background: The transcriptional repressor BMI-1 was first identified as a proto-oncogene frequently activated by Moloney murine leukemia proviral insertions in mice and cooperating with c-myc in the generation of mouse lymphomas . BMI-1 is involved in segment specification, cell growth and maintenance, transcriptional regulation, and chromatin modification. A major target of BMI-1 is the ink4a locus which encodes tumor suppressor proteins p16 and p19Arf, which are important in tumor progression and thought to be critical in cell proliferation and senescence (2). Recent studies have also shown that BMI-1 is required for the maintenance of adult normal and leukemic stem cells (3), suggesting that BMI-1 could an attractive therapeutic target for stem cell proliferation and renewal as well as for anti-cancer strategies.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy