Carabin antibody (C-Term)

Catalog No. ABIN499529

The Rabbit Polyclonal anti-Carabin antibody is suitable to detect Carabin in samples from Human and Mouse. It has been validated for WB, IHC (p) and EIA.

Quick Overview for Carabin antibody (C-Term) (ABIN499529)

Target: Carabin (TBC1D10C) (TBC1 Domain Family, Member 10C (TBC1D10C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Carabin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Carabin Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to Carabin.

Purification: Affinity chromatography purified via peptide column

Immunogen: Carabin antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human Carabin.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Carabin antibody can be used for detection of Carabin at 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Carabin

Target: Carabin (TBC1D10C) (TBC1 Domain Family, Member 10C (TBC1D10C))

Alternative Name: Carabin

Background: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.Synonyms: TBC1 domain family member 10C, TBC1D10C

Gene ID: 374403

NCBI Accession: NP_940919

UniProt: Q8IV04