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Caspase 12 (CASP12) (large) antibody

Details for Product No. ABIN499551, Supplier: Log in to see
Antigen
  • Caspase-12
Epitope
large
16
15
15
13
12
11
9
7
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
101
86
62
Host
Rabbit
127
11
10
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
127
60
51
35
15
12
11
6
6
5
2
1
Supplier
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Immunogen Mouse Caspase-12 (Large) Peptide
Isotype IgG
Specificity Caspase-12 antibody was raised against a synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12.
Purification Affinity chromatography purified via peptide column
Alternative Name Caspase-12 (CASP12 Antibody Abstract)
Background Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including beta-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues (1).Synonyms: CASP-12, CASP12, Inactive caspase-12
Gene ID 100044205
UniProt O08736
Research Area Apoptosis/Necrosis
Application Notes ELISA. Western Blot: Caspase-12 (large) antibody can be used for the detection of both thepropeptide and cleaved forms of caspase-12 at 0.5 to 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Caspase 12 (CASP12) (large) antibody (ABIN499551) Immunohistochemical staining of human heart tissue using AP30192PU-N caspase-12 antib...
Western Blotting (WB) image for anti-Caspase 12 (CASP12) (large) antibody (ABIN499551) Western blot analysis of caspase-12 in human heart lysate with AP30192PU-N caspase-12...
Background publications Nakagawa, Zhu, Morishima et al.: "Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta." in: Nature, Vol. 403, Issue 6765, pp. 98-103, 2000 (PubMed).

Mehmet: "Caspases find a new place to hide." in: Nature, Vol. 403, Issue 6765, pp. 29-30, 2000 (PubMed).

Van de Craen, Vandenabeele, Declercq et al.: "Characterization of seven murine caspase family members." in: FEBS letters, Vol. 403, Issue 1, pp. 61-9, 1997 (PubMed).