Caspase 4 antibody (Intermediate Domain)

Catalog No. ABIN499563

This anti-Caspase 4 antibody is a Rabbit Polyclonal antibody detecting Caspase 4 in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for Caspase 4 antibody (Intermediate Domain) (ABIN499563)

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Caspase 4 Antibody

Binding Specificity: Intermediate Domain

Specificity: Caspase-4 antibody was raised against a 15 amino acid peptide from near the center of human Caspase-4.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human Casp-4 / ICH-2 / ICE rel II / Mih1 (Intermediate Domain) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: Casp-4 antibody can be used for the detection of Caspase-4 at 2 μg/mL. Depending on cell lines or tissues used, other cleavage products may be observed. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for Caspase 4

Target: Caspase 4 (CASP4) (Caspase 4, Apoptosis-Related Cysteine Peptidase (CASP4))

Alternative Name: Caspase-4

Background: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens (reviewed in 1). Members of this subfamily include caspase-1, -4, -5, and -12. Activation of these caspases results in the cleavage and activation of proinflammatory cytokines such as IL-1beta and IL-18 (2,3). Caspase-4 was initially identified as a homologous protein to Caspase-1 and the C. elegans Ced-3 which could induce apoptosis in transfected cells (4). More recent studies have shown that it can be activated by ER stress and has been suggested to be involved in multiple neuronal pathologies such as Alzheimers disease (5).Synonyms: CASP4, ICE(rel)-II, ICH2, Protease ICH-2, Protease TX

Gene ID: 837

UniProt: P49662

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity