Checkpoint Kinase 2 (CHEK2) (N-Term) antibody

Details for Product No. ABIN499619
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Antigen
Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
Epitope
N-Term
(39), (38), (30), (29), (17), (13), (13), (11), (9), (7), (6), (6), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(350), (146), (145), (55), (37), (36), (12), (7), (6), (1)
Host
Rabbit
(284), (73), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(275), (109), (96), (67), (60), (46), (35), (31), (16), (4), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499619
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Immunogen Human Chk2 (N-Terminus) Peptide
Isotype IgG
Specificity Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
Purification Affinity chromatography purified via peptide column
Alternative Name CHK2
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).
Alternate names: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_009125
UniProt O96017
Application Notes ELISA. Western Blot: Chk2 antibody can be used for detection of Chk2 at 0.5 to 1 μg/ml. A 60 kDaband can be detected. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS containing 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8°C.
Expiry Date 12 months
Background publications Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa et al.: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

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