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EBI3 antibody (Epstein-Barr Virus Induced 3) (Center)

Details for Product anti-EBI3 Antibody No. ABIN499778, Supplier: Log in to see
Antigen
  • IL-27B
  • IL27B
  • EBI-3
  • IL-27
  • Epstein-Barr virus induced 3
  • Epstein-Barr virus induced gene 3
  • EBI3
  • Ebi3
Epitope
Center
17
15
9
8
6
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
98
55
25
1
1
1
1
1
1
Host
Rabbit
87
14
11
8
Clonality
Polyclonal
Conjugate
This EBI3 antibody is un-conjugated
5
5
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
95
57
26
16
13
7
5
4
2
2
1
1
1
Options
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Immunogen EBI3 antibody was raised against a 16 amino acid peptide from near the center of human EBI3.
Isotype IgG
Specificity This antibody reacts to EBI3.
Purification Affinity chromatography purified via peptide column
Alternative Name EBI3 / IL27B (EBI3 Antibody Abstract)
Background EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35 (1-3). Like interleukin-23 (IL-23), IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naive but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-g, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.Synonyms: EBV-induced gene 3 protein, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta, IL-27B, Interleukin-27 subunit beta
Gene ID 10148
NCBI Accession NP_005746
UniProt Q14213
Application Notes ELISA. Western Blot: 2 - 4 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-EBI3 antibody (Epstein-Barr Virus Induced 3) (Center) (ABIN499778) Immunohistochemistry of EBI3 in human spleen tissue with EBI3 antibody at 2.5 μg/ml.
Western Blotting (WB) image for anti-EBI3 antibody (Epstein-Barr Virus Induced 3) (Center) (ABIN499778) Western blot analysis of EBI3 in rat spleen tissue lysate with AP30307PU-N EBI3 antib...
Background publications Niedbala, Wei, Cai, Hueber, Leung, McInnes, Liew: "IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells." in: European journal of immunology, Vol. 37, Issue 11, pp. 3021-9, 2007 (PubMed).

Pflanz, Hibbert, Mattson, Rosales, Vaisberg, Bazan, Phillips, McClanahan, de Waal Malefyt, Kastelein: "WSX-1 and glycoprotein 130 constitute a signal-transducing receptor for IL-27." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 4, pp. 2225-31, 2004 (PubMed).

Pflanz, Timans, Cheung, Rosales, Kanzler, Gilbert, Hibbert, Churakova, Travis, Vaisberg, Blumenschein, Mattson, Wagner, To, Zurawski, McClanahan, Gorman, Bazan, de Waal Malefyt, Rennick, Kastelein: "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4+ T cells." in: Immunity, Vol. 16, Issue 6, pp. 779-90, 2002 (PubMed).

Devergne, Birkenbach, Kieff: "Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 22, pp. 12041-6, 1997 (PubMed).