IFIH1 antibody (Center)

Catalog No. ABIN500266

This Rabbit Polyclonal antibody specifically detects IFIH1 in WB, IF, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for IFIH1 antibody (Center) (ABIN500266)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-IFIH1 Antibody

Binding Specificity: Center

Specificity: This antibody detects Helicard at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse

Purification: Peptide Affinity Chromatography

Immunogen: 16 amino acid peptide from near the center of Human MDA5.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: Helicard

Background: The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the melanoma differentiation-associated protein 5 (MDA5) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. MDA5, a member of the DEAD/DEAH-box RNA helicase family, consists of an amino-terminal caspase recruitment domain (CARD) and a carboxyl-terminal RNA helicase domain similar to that of the related protein RIG-1. When stimulated by dsRNA, MDA5 recruits the adaptor protein VISA and ultimately causes the activation of IRF-3 and NF-kB. MDA5 and RIG-1 recognize different types of dsRNA, with MDA5 recognizing poly (I:C). MDA5-null mice were highly susceptible to infection with picornaviruses, which possess such sequences, demonstrating the importance of MDA5 in innate immunity.Synonyms: Helicase with 2 CARD domains, IFIH1, Interferon-induced helicase C domain-containing protein 1, MDA5, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RH116, RNA helicase-DEAD box protein 116

Gene ID: 64135

NCBI Accession: NP_071451

UniProt: Q9BYX4

Pathways: Activation of Innate immune Response