Nanos Homolog 1 antibody (Center)

Catalog No. ABIN500318

The Rabbit Polyclonal anti-Nanos Homolog 1 antibody is suitable to detect Nanos Homolog 1 in samples from Human, Mouse and Rat. It has been validated for WB and EIA.

Quick Overview for Nanos Homolog 1 antibody (Center) (ABIN500318)

Target: Nanos Homolog 1 (NANOS1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nanos Homolog 1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Nanos Homolog 1 Antibody

Binding Specificity: Center

Specificity: This antibody detects NANOS1 at center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: Nanos1 antibody was raised against a 14 amino acid peptide from near the center of human Nanos1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Nanos Homolog 1

Target: Nanos Homolog 1 (NANOS1)

Alternative Name: NANOS1

Background: Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.Synonyms: NOS1, Nanos homolog 1

Gene ID: 340719

UniProt: Q8WY41

Pathways: Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction