PICALM antibody (N-Term)

Catalog No. ABIN500493

The Rabbit Polyclonal anti-PICALM antibody (ABIN500493) specifically detects PICALM in WB and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PICALM antibody (N-Term) (ABIN500493)

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PICALM antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PICALM Antibody

Binding Specificity: N-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: PICALM antibody was raised against a 15 amino acid peptide from near the amino terminus of human PICALM.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PICALM

Target: PICALM (Phosphatidylinositol Binding clathrin Assembly Protein (PICALM))

Alternative Name: PICALM

Background: PICALM, also known as the clathrin assembly lymphoid myeloid leukemia (CALM) protein, co-localizes with clathrin at clathrin-coated areas of the plasma membrane and in the Golgi area, and is thought to be an important component of the coated pit internalization machinery. Fusion proteins involving PICALM have been implicated in acute myeloid and T-cell acute lymphoblastic leukemia. PICALM is also expressed in neurons and is present at the synapse where it facilitates the endocytosis of the synaptic vesicle protein VAMP2. Recent studies have identified variants of PICALM associated with Alzheimer's disease and have suggested this protein may play a role in the endocytosis and recycling of the amyloid protein precursor (APP).Synonyms: CALM, Clathrin assembly lymphoid myeloid leukemia protein, Phosphatidylinositol-binding clathrin assembly protein

Gene ID: 8301

NCBI Accession: NP_009097

UniProt: Q13492

Pathways: Synaptic Membrane