CRADD antibody (Middle Region)

Catalog No. ABIN500561

The Rabbit Polyclonal anti-CRADD antibody is suitable to detect CRADD in samples from Human. It has been validated for WB, IF and EIA.

Quick Overview for CRADD antibody (Middle Region) (ABIN500561)

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRADD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-CRADD Antibody

Binding Specificity: AA 99-117, Middle Region

Specificity: This antibody detects RAIDD at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Peptide Affinity Chromatography

Immunogen: RAIDD antibody was raised against a peptide corresponding to amino acids 99 to 117 of human RAIDD.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1: 500 - 1: 1000, a 22 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRADD

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: RAIDD

Background: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain (DD)-containing receptors, TNFR1 and Fas. The death signals are transduced by a group of DD-containing adapter molecules. A novel cell death adapter was recently identified by two independent groups and designated RAIDD (RIP-associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD)1,RAIDD contains a DD and a CARD (for caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals1,3. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR-1.Synonyms: CRADD, Caspase and RIP adapter with death domain, Death domain-containing protein CRADD, RIP-associated protein with a death domain

Gene ID: 8738

UniProt: P78560

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity