SATB1 antibody (C-Term)

Catalog No. ABIN500670

The Rabbit Polyclonal anti-SATB1 antibody (ABIN500670) specifically detects SATB1 in WB, IHC (p) and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SATB1 antibody (C-Term) (ABIN500670)

Target: SATB1 (SATB Homeobox 1 (SATB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SATB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-SATB1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects SATB1 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: SATB1 antibody was raised against a 15 amino acid peptide near the carboxy terminus of the human SATB1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 2 - 4 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Alternative Name: SATB1

Background: Human special AT-rich sequence-binding protein-1 (SATB1) is a nuclear matrix/scaffold-associated region DNA-binding protein, predominantly expressed in the thymus and pre-B cells. Like its homolog SATB2, SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which one strand exclusively consists of well-mixed A, T, and C nucleotides. SATB1 contains a dimerization domain that shares similarity with the PDZ motif, identified as an indispensable element for high-affinity binding of SATB1 to DNA. SATB1 constitutes a functional nuclear architecture that has a \'cage-like\' protein distribution surrounding heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex) and transcription factors recruitment. SATB1 functions as a \'genome organizer\' essential for proper T-cell development. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic, and when ectopically expressed in non-metastatic cells, can induce invasive activity in vivo. At least two isoforms of SATB1 are known to exist.Synonyms: DNA-binding protein SATB1, Special AT-rich sequence-binding protein 1

Gene ID: 6304

UniProt: Q01826

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation