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Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody

Details for Product No. ABIN500827, Supplier: Log in to see
Antigen
  • stim1
  • MGC76045
  • STIM1
  • si:dkey-24p1.5
  • MGC154748
  • si:ch211-112l17.2
  • Stim1
  • D11S4896E
  • GOK
  • TAM
  • SIM
Epitope
C-Term
35
14
7
7
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
104
39
38
3
3
1
1
Host
Rabbit
67
24
11
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
90
53
40
27
12
12
11
5
3
1
1
Supplier
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Immunogen STIM1 antibody was raised against a 24 amino acid peptide from near the carboxy terminus of human STIM1.
Isotype IgG
Specificity This antibody detects STIM1 / GOK. It is predicted to have no cross-reactivity to STIM2.
Purification Peptide affinity chromatography
Alternative Name STIM1 / GOK (STIM1 Antibody Abstract)
Background In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least two isoforms of STIM1 are known to exist, this antibody will detect only the larger form.Synonyms: Stromal interaction molecule 1
Gene ID 6786
NCBI Accession NP_003147
Pathways
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunohisochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody (ABIN500827) Immunohistochemistry of STIM1 in human spleen tissue with this product at 2.5 μg/ml.
Western Blotting (WB) image for anti-Stromal Interaction Molecule 1 (STIM1) (C-Term) antibody (ABIN500827) Western blot analysis of STIM1 in mouse thymus tissue lysate with this product at (A)...
Background publications Luik, Lewis: "New insights into the molecular mechanisms of store-operated Ca2+ signaling in T cells." in: Trends in molecular medicine, Vol. 13, Issue 3, pp. 103-7, 2007 (PubMed).

Feske, Gwack, Prakriya et al.: "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function." in: Nature, Vol. 441, Issue 7090, pp. 179-85, 2006 (PubMed).

Spassova, Soboloff, He et al.: "STIM1 has a plasma membrane role in the activation of store-operated Ca(2+) channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 11, pp. 4040-5, 2006 (PubMed).

Zhang, Yu, Roos et al.: "STIM1 is a Ca2+ sensor that activates CRAC channels and migrates from the Ca2+ store to the plasma membrane." in: Nature, Vol. 437, Issue 7060, pp. 902-5, 2005 (PubMed).