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ADAM17 antibody (ADAM Metallopeptidase Domain 17) (C-Term)

Details for Product anti-ADAM17 Antibody No. ABIN500861, Supplier: Login to see
Antigen
  • TACE
  • adam17
  • si:dkey-81b15.2
  • adam18
  • cd156b
  • csvp
  • tace
  • CD156b
  • Tace
  • ADAM18
  • CD156B
  • CSVP
  • NISBD
Alternatives
anti-Human ADAM17 antibody for Western Blotting
Epitope
C-Term
84
18
14
14
14
10
7
5
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
213
65
56
2
2
1
1
1
1
1
Host
Rabbit
176
27
16
1
1
Clonality
Polyclonal
Conjugate
This ADAM17 antibody is un-conjugated
9
8
6
6
5
5
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
180
121
27
26
21
14
13
8
7
4
3
2
1
1
Supplier
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Immunogen A peptide corresponding to amino acids near the carboxy terminus of Human CD156b / ADAM17
Isotype IgG
Specificity This antibody detects CD156b / ADAM17 at C-term. 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated CD156b.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat.
Purification Immunoaffinity Chromatography
Alternative Name CD156b / ADAM17 (ADAM17 Antibody Abstract)
Background Tumor-necrosis factor-a is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-alpha is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-alpha. TNF-alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFa, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.Synonyms: CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, TACE, TNF-alpha convertase, TNF-alpha-converting enzyme
Gene ID 6868
NCBI Accession NP_003174
Research Area CD Antigens, Surface Receptors of Immune Cells, Enzymes
Pathways Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 1: 500 to 1: 2000. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-ADAM17 antibody (ADAM Metallopeptidase Domain 17) (C-Term) (ABIN500861) Immunocytochemistry of TACE in HeLa cells with TACE Antibody AP30854PU-N at 10 µg/ml.
Immunofluorescence (IF) image for anti-ADAM17 antibody (ADAM Metallopeptidase Domain 17) (C-Term) (ABIN500861) Immunofluorescence of TACE in HeLa cells with TACE at 10 µg/ml.
Western Blotting (WB) image for anti-ADAM17 antibody (ADAM Metallopeptidase Domain 17) (C-Term) (ABIN500861) Western blot analysis of TACE in HeLa (1,3) and Jurkat (2,4) whole cell lysate in the...
Background publications Mizui, Yamazaki, Sagane et al.: "cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter." in: Gene, Vol. 233, Issue 1-2, pp. 67-74, 1999 (PubMed).

Peschon, Slack, Reddy et al.: "An essential role for ectodomain shedding in mammalian development." in: Science (New York, N.Y.), Vol. 282, Issue 5392, pp. 1281-4, 1998 (PubMed).

Buxbaum, Liu, Luo et al.: "Evidence that tumor necrosis factor alpha converting enzyme is involved in regulated alpha-secretase cleavage of the Alzheimer amyloid protein precursor." in: The Journal of biological chemistry, Vol. 273, Issue 43, pp. 27765-7, 1998 (PubMed).

Giegel, Chaplin: "Targets in cytokine activation." in: Agents and actions. Supplements, Vol. 49, pp. 1-3, 1998 (PubMed).

Moss, Jin, Milla et al.: "Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. ..." in: Nature, Vol. 385, Issue 6618, pp. 733-6, 1997 (PubMed).

Black, Rauch, Kozlosky et al.: "A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells." in: Nature, Vol. 385, Issue 6618, pp. 729-33, 1997 (PubMed).