TWEAK antibody

Catalog No. ABIN501094

This Rabbit Polyclonal antibody specifically detects TWEAK in WB, EIA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TWEAK antibody (ABIN501094)

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TWEAK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TWEAK Antibody

Specificity: This antibody detects TWEAK / TNFSF12.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Peptide Affinity Chromatography

Immunogen: Recombinant Human TWEAK.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TWEAK

Target: TWEAK (TNFSF12) (Tumor Necrosis Factor (Ligand) Superfamily, Member 12 (TNFSF12))

Alternative Name: TWEAK / TNFSF12

Background: TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor superfamily (TNFSF) of structurally related cytokines. Like most other members of this family, TWEAK is a cell surface-associated type II transmembrane protein although a smaller, biologically active form can also be generated by cleavage near the cell membrane (1,2). TWEAK has multiple biological activities, including stimulation of cell growth and angiogenesis (3), induction of inflammatory cytokines (4), in addition to stimulation of apoptosis (5). The TWEAK signal transduction pathway has not been well established but it appears to signal via TweakR/Fn14 in a manner similar to that described for other TNFSF members that bind receptors lacking death domains (1). Despite its predicted molecular weight of 27 kDa, TWEAK may migrate at aberrant positions in SDS-PAGE.Synonyms: APO3 ligand, APO3L, DR3LG, TNF-related weak inducer of apoptosis, Tumor necrosis factor ligand superfamily member 12

Gene ID: 8742

NCBI Accession: NP_003800

Pathways: Apoptosis