Myocyte Enhancer Factor 2A (MEF2A) antibody

Antigen: Myocyte Enhancer Factor 2A (MEF2A)

Synonyms: ADCAD1, RSRFC4, RSRFC9, A430079H05Rik, wu:fd19d02, MEF2A, DKFZp469F0316, mef2a, MGC52609, xmef2a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine), Human, Rat (Rattus), Mouse (Murine), Zebrafish, Dog (Canine), Xenopus laevis, Pig (Porcine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN501252

Additional Information

Alternative name: MEF2A

Gene ID: MEF2A

UniProt: Q02078

Immunogen: The immunogen for anti-MEF2A antibody: synthetic peptide directed towards the N terminal of human MEF2A

Sequence: YNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHT EEKYKKINEE

Cross-Reactivity: Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-MEF2A antibody concentration is 1 mg/ml.

Description: The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD, myogenin, MYF5, and MRF4 are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes.The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Characteristics: Antigen Size: 507 amino acids

Molecular Weight: 55kDa

Application Details

Application Notes: WB Suggested Anti-MEF2A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Transfected 293T.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Research Area: Cardiovascular, Hypertrophy, Cardiogenesis, Transcription Factors, Neurogenesis

Restrictions: For Research Use only

Images

WB Suggested Anti-MEF2A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Transfected 293T

Publications

Product: Ramachandran, Yu, Gulick: "Nuclear respiratory factor 1 controls myocyte enhancer factor 2A transcription to provide a mechanism for coordinate expression of respiratory chain subunits." in: The Journal of biological chemistry, Vol. 283, Issue 18, pp. 11935-46, 2008 (PubMed).