Gap Junction Protein, delta 4, 40.1kDa (GJD4) (Middle Region) antibody

Details for Product No. ABIN501575
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Antigen
Synonyms 9430022F06Rik, Cx39, CX40.1, RP11-425A6.2
Epitope
Middle Region
(3), (1), (1)
Reactivity
Human
(6)
Host
Rabbit
(5), (1)
Clonality
Polyclonal
Application
Western Blotting (WB)
(6), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN501575
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Immunogen Synthetic peptide directed towards the middle region of human GJD4
Sequence HTKIPDEDES EVTSSASEKL GRQPRGRPHR EAAQDPRGSG SEEQPSAAPS
Predicted Reactivity Human :100 %,   Crab-eating macaque :90 %,  
Characteristics This is a rabbit polyclonal antibody against GJD4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name GJD4
Background Connexins, such as GJD4, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin s
Molecular Weight 40 kDa
Gene ID 219770
NCBI Accession NP_699199, NM_153368
UniProt Q96KN9
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 370 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Gap Junction Protein, delta 4, 40.1kDa (GJD4) (Middle Region) antibody WB Suggested Anti-GJD4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: PANC1 cell lysate
Product cited in: Terrier-Lacombe, Guillou, Maire et al.: "Dermatofibrosarcoma protuberans, giant cell fibroblastoma, and hybrid lesions in children: clinicopathologic comparative analysis of 28 cases with molecular data--a study from the French Federation of Cancer Centers Sarcoma Group." in: The American journal of surgical pathology, Vol. 27, Issue 1, pp. 27-39, 2002 (PubMed).

Background publications Söhl, Nielsen, Eiberger et al.: "Expression profiles of the novel human connexin genes hCx30.2, hCx40.1, and hCx62 differ from their putative mouse orthologues." in: Cell communication & adhesion, Vol. 10, Issue 1, pp. 27-36, 2003 (PubMed).

Validation Images
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