Polymerase (DNA-Directed), epsilon 4 (p12 Subunit) (POLE4) (N-Term) antibody

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Antigen
Synonyms 2400007P05Rik, 5830430F06Rik, YHHQ1, p12
Alternatives
Epitope
N-Term
(2), (1), (1), (1)
Reactivity
Cow (Bovine), Dog (Canine), Human
(10), (5), (1), (1)
Host
Rabbit
(9), (1)
Clonality
Polyclonal
Application
Western Blotting (WB)
(8), (6), (3)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human POLE4
Blocking Peptide Blocking peptide for this product available: Polymerase (DNA-Directed), epsilon 4 (p12 Subunit) (POLE4) (N-Term) peptide (ABIN981949)
Sequence MAAAAAAGSG TPREEEGPAG EAAASQPQAP TSVPGARLSR LPLARVKALV
Predicted Reactivity Dog : 92 %, Guinea pig : 92 %, Horse : 82 %, Rabbit : 72 %
Characteristics This is a rabbit polyclonal antibody against POLE4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name POLE4 (POLE4 Antibody Abstract)
Background POLE4 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Molecular Weight 12 kDa
Gene ID 56655
NCBI Accession NP_063949, NM_019896
UniProt Q9NR33
Pathways DNA Damage Repair
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 117 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Polymerase (DNA-Directed), epsilon 4 (p12 Subunit) (POLE4) (N-Term) antibody (ABIN501810) WB Suggested Anti-POLE4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Background publications Hillier, Graves, Fulton et al.: "Generation and annotation of the DNA sequences of human chromosomes 2 and 4. ..." in: Nature, Vol. 434, Issue 7034, pp. 724-31, 2005 (PubMed).

Catalog No. ABIN501810
$289.00
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