Actin, beta (ACTB) (Middle Region) antibody

Details for Product No. ABIN501930
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Antigen
Synonyms
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Ba ... show more
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, BRWS1, PS1TP5BP1, ACT-5, Actx, E430023M04Rik, ACTA1, Beta-actin, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, POTE2alpha, POTEACTIN show less
Epitope
Middle Region
(116), (36), (12), (9), (6), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(383), (194), (179), (67), (49), (44), (40), (33), (30), (26), (22), (18), (12), (11), (10), (9), (7), (7), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host
Rabbit
(246), (151), (22)
Clonality
Polyclonal
Conjugate
Un-conjugated
(20), (16), (10), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(374), (172), (96), (91), (68), (53), (31), (29), (20), (18), (12), (9), (9), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN501930
289.00 $
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Immunogen The immunogen for Anti-ACTB antibody is: synthetic peptide directed towards the middle region of Human ACTB
Sequence TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Sheep : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.
Purification Affinity Purified
Alternative Name ACTB
Background This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Molecular Weight 42 kDa
Gene ID 60
NCBI Accession NP_001092, NM_001101
UniProt P60709
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 375 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Actin, beta (ACTB) (Middle Region) antibody WB Suggested Anti-ACTB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Transfected 293T
anti-Actin, beta (ACTB) (Middle Region) antibody (2) Sample type: 1-8.N2 WT C elegans (60ug)
Primary Dilution: 1:2000
Secondary Antibody: goat anti-Rabbit HRP
Secondary Dilution: 1:2000

anti-Actin, beta (ACTB) (Middle Region) antibody (3) Sample Type: NT2 cells
Red: Antibody
Blue: DAPI
Primary Dilution: 1ug/50ul antibody
Secondary Antibody: Alexa goat anti-rabbit 594
Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences
anti-Actin, beta (ACTB) (Middle Region) antibody (4) Host: Rabbit
Target Name: ACTB
Sample Tissue: HepG2Lane A: Primary Antibody Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1 µg/mL Peptide Concentration: 5.0 µg/mL Lysate Quantity:.41 µg/lane/laneGel Concentration: 12 %
anti-Actin, beta (ACTB) (Middle Region) antibody (5) WB Suggested Anti-ACTB Antibody
Positive Control: Lane 1: 341 µg mouse 3T3 ECM extract + blocking peptide, Lane 2: 041 µg mouse 3T3 ECM extract, Lane 3: 041 µg Echinococcus granulosus extract
Primary Antibody Dilution: 1:000
Secondary Antibody: Anti-rabbit-HRP Secondry
Antibody Dilution: 1:00,000
Submitted by: Anonymous
Product cited in: Rinne, Clements, Lamme et al.: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, 2008 (PubMed).

Validation Images
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