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beta Actin antibody (Actin, beta) (Middle Region)

Details for Product anti-ACTB Antibody No. ABIN501930, Supplier: Login to see New
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Antigen
Synonyms
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Ba ... show more
beta-actin, ps1tp5bp1, LOC563571, ACTB, A26C1A, A26C1B, act-2, actb, B-actin, LOC100136013, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, BRWS1, PS1TP5BP1, ACT-5, Actx, E430023M04Rik, ACTA1, Beta-actin, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, POTE2alpha, POTEACTIN show less
Epitope
Middle Region
(133), (38), (15), (14), (11), (7), (6), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(486), (190), (167), (52), (51), (40), (34), (32), (30), (23), (18), (14), (12), (8), (8), (7), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(313), (202), (19), (1), (1)
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
(27), (19), (15), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(460), (212), (145), (90), (85), (48), (42), (20), (12), (12), (11), (11), (9), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen The immunogen for Anti-ACTB antibody is: synthetic peptide directed towards the middle region of Human ACTB
Blocking Peptide Blocking peptide for this product available: ABIN972858
Sequence TMKIKIIAPP ERKYSVWIGG SILASLSTFQ QMWISKQEYD ESGPSIVHRK
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Sheep : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against ACTB. It was validated on Western Blot.
Purification Affinity Purified
Alternative Name ACTB (ACTB Antibody Abstract)
Background This protein is one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Molecular Weight 42 kDa
Gene ID 60
NCBI Accession NP_001092, NM_001101
UniProt P60709
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 375 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (Middle Region) (ABIN501930) WB Suggested Anti-ACTB Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (Middle Region) (ABIN501930) Sample type: 1-8.N2 WT C elegans (60ug)
Primary Dilution: 1:2000
...
Immunohistochemistry (IHC) image for anti-beta Actin antibody (Actin, beta) (Middle Region) (ABIN501930) Sample Type: NT2 cells
Red: Antibody
Blue: DAPI
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (Middle Region) (ABIN501930) Host: Rabbit
Target Name: ACTB
Sample Tissue: HepG2Lane A: Primary Ant...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (Middle Region) (ABIN501930) WB Suggested Anti-ACTB Antibody
Positive Control: Lane 1: 341 µg mouse 3T3 ECM...
Product cited in: Rinne, Clements, Lamme et al.: "A novel translation re-initiation mechanism for the p63 gene revealed by amino-terminal truncating mutations in Rapp-Hodgkin/Hay-Wells-like syndromes." in: Human molecular genetics, Vol. 17, Issue 13, pp. 1968-77, 2008 (PubMed).

Catalog No. ABIN501930
$289.00
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