RNP antibody (Middle Region)

Catalog No. ABIN502034

The Rabbit Polyclonal anti-RNP antibody is suitable to detect RNP in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for RNP antibody (Middle Region) (ABIN502034)

Target: RNP (RNPC3) (RNA-Binding Region (RNP1, RRM) Containing 3 (RNPC3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNP antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RNP Antibody

Binding Specificity: Middle Region

Purpose: RNPC3 Antibody - middle region

Sequence: LHAPLPPTSP QPPEEPPLPD EDEELSSEES EYESTDDEDR QRMNKLMELA

Predicted Reactivity: Cow: 92%, Dog: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Pig: 92%, Rabbit: 92%, Rat: 85%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against RNPC3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RNPC3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP40868-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for RNP

Target: RNP (RNPC3) (RNA-Binding Region (RNP1, RRM) Containing 3 (RNPC3))

Alternative Name: RNPC3

Background:

Background Information: RNPC3 is an RNA-binding protein involved in pre-mRNA splicing. RNPC3 participates in pre-mRNA U12-dependent splicing. RNPC3 binds to the 3'-stem-loop of m7G-capped U12 snRNA.Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99 % of pre-mRNA introns. The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. This gene encodes a 65K protein that is a component of the U12-type spliceosome. This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome.

Gene Name: RNA-binding region (RNP1, RRM) containing 3

Alternative Symbols: RNP, IGHD5, RBM40, SNRNP65

Protein Name: RNA-binding protein 40

Molecular Weight: 58kDa

Gene ID: 55599

NCBI Accession: NP_060089

UniProt: Q96LT9