Centromere Protein A (CENPA) (Middle Region) antibody

Details for Product No. ABIN502692
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Antigen
Synonyms
BcDNA:RE21270, CENP-A, CENP-A/CID, CENP-A/Cid, CENP-C, CENPA, CG13329, CID, CID/CENP-A, CenH3, CenH3/CID, CenH3[CID], CenH3[Cid], Cenp-A, CenpA, CenpA/CID, Cid, Cid[Mel], Dmel\\CG13329, cenH3, cenpA,  ... show more
BcDNA:RE21270, CENP-A, CENP-A/CID, CENP-A/Cid, CENP-C, CENPA, CG13329, CID, CID/CENP-A, CenH3, CenH3/CID, CenH3[CID], CenH3[Cid], Cenp-A, CenpA, CenpA/CID, Cid, Cid[Mel], Dmel\\CG13329, cenH3, cenpA, cenpx, cenp-a, sim2, Cenp-a, RGD1563607 show less
Epitope
Middle Region
(20), (14), (7), (7), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus), Mouse (Murine), Cow (Bovine), Zebrafish (Danio rerio), Human
(95), (14), (12), (12), (12), (12), (1)
Host
Rabbit
(67), (26), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(60), (34), (20), (17), (13), (9), (5), (2), (1), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN502692
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Immunogen Synthetic peptide directed towards the middle region of human CENPA
Sequence ALQEAAEAFL VHLFEDAYLL TLHAGRVTLF PKDVQLARRI RGLEEGLG
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Rat : 100 %, Mouse : 80 %, Chicken : 80 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against CENPA. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name CENPA
Background Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Molecular Weight 16 kDa
Gene ID 1058
NCBI Accession NM_001809, NP_001800
UniProt P49450
Research Area Chromatin and Nuclear Signaling, Cell Cycle
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 140 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Centromere Protein A (CENPA) (Middle Region) antibody WB Suggested Anti-CENPA Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Hela cell lysate
anti-Centromere Protein A (CENPA) (Middle Region) antibody (2) Host: Rabbit
Target Name: CENPA
Sample Tissue: 721_B
Antibody Dilution: 1.0 µg/mL CENPA is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
Background publications Orthaus, Biskup, Hoffmann et al.: "Assembly of the inner kinetochore proteins CENP-A and CENP-B in living human cells." in: Chembiochem : a European journal of chemical biology, Vol. 9, Issue 1, pp. 77-92, 2007 (PubMed).

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