Mitochondrial Ribosomal Protein L15 (MRPL15) (C-Term) antibody

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Antigen
Synonyms L15mt, MRP-L15, MRP-L7, RPML7, HSPC145, Rpml7, zgc:92856
Epitope
C-Term
(6), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(32), (14), (7), (2)
Host
Rabbit
(25), (7)
Clonality
Polyclonal
Application
Western Blotting (WB)
(28), (11), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the C terminal of human MRPL15
Blocking Peptide Blocking peptide for this product available: Mitochondrial Ribosomal Protein L15 (MRPL15) peptide (ABIN980244)
Sequence KDELFKMLCT RKDPRQIFFG LAPGWVVNMA DKKILKPTDE NLLKYYTS
Predicted Reactivity Dog : 100 %, Human : 100 %, Pig : 100 %, Rat : 100 %, Chicken : 90 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against MRPL15. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MRPL15 (MRPL15 Antibody Abstract)
Background Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL15 belongs to the ribosomal protein L15P family. It is a 39S subunit protein that belongs to the EcoL15 ribosomal protein family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.
Molecular Weight 33 kDa
Gene ID 29088
NCBI Accession NP_054894, NM_014175
UniProt Q9P015
Research Area Chromatin and Nuclear Signaling, DNA/RNA, Organelles
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 296 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Mitochondrial Ribosomal Protein L15 (MRPL15) (C-Term) antibody (ABIN502725) WB Suggested Anti-MRPL15 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Product cited in: Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Catalog No. ABIN502725
$289.00
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