MRPL15 antibody (N-Term)

Catalog No. ABIN2782532

There is 1 publication for this product available. The Rabbit Polyclonal anti-MRPL15 antibody is suitable to detect MRPL15 in samples from Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig and Horse. It has been validated for WB.

Quick Overview for MRPL15 antibody (N-Term) (ABIN2782532)

Target: MRPL15 (Mitochondrial Ribosomal Protein L15 (MRPL15))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL15 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MRPL15 Antibody

Binding Specificity: N-Term

Purpose: MRPL15 Antibody - N-terminal region

Sequence: KPERRPRGRR RGRKCGRGHK GERQRGTRPR LGFEGGQTPF YIRIPKYGFN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MRPL15. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL15

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP46191-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-MRPL15 antibody (ABIN2782532)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for MRPL15

Target: MRPL15 (Mitochondrial Ribosomal Protein L15 (MRPL15))

Alternative Name: MRPL15

Background:

Background Information: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL15 belongs to the ribosomal protein L15P family. It is a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.

Gene Name: Mitochondrial ribosomal protein L15

Alternative Symbols: L15mt, RPML7, MRP-L7, HSPC145, MRP-L15

Protein Name: 39S ribosomal protein L15, mitochondrial

Molecular Weight: 33kDa

Gene ID: 29088

NCBI Accession: NP_054894

UniProt: Q9P015