Hsc70 antibody (N-Term)

Catalog No. ABIN502949

The Rabbit Polyclonal anti-Hsc70 antibody has been validated for WB and IHC. It is suitable to detect Hsc70 in samples from Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio) and Goat.

Quick Overview for Hsc70 antibody (N-Term) (ABIN502949)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Cow, Sheep, Pig, Zebrafish (Danio rerio), Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Hsc70 Antibody

Binding Specificity: N-Term

Sequence: MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL

Predicted Reactivity: Cow: 100%, Goat: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against HSPA8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSPA8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 646 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8

Background: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.
Alias Symbols: HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP1, MGC131511, MGC29929, NIP71
Protein Interaction Partner: FUS, HSPBP1, TRIM38, STUB1, ISG15, UBC, TP53, BAG1, BAG3, IL32, NMI, AIRE, HAUS2, TUBGCP4, CEP250, TUBGCP2, TUBGCP3, TUBG1, AURKA, SUMO2, SUMO3, MDM2, LGALS3BP, CDKN1A, CDC20, HAUS1, STAU1, ST13, HSPA4, HSPA2, DNAJA1, GABRB1, EDRF1, DNAJC9, HSPA4L, HSPH1,
Protein Size: 646

Molecular Weight: 71 kDa

Gene ID: 3312

NCBI Accession: NM_006597, NP_006588

UniProt: P11142