B3GALTL antibody (Middle Region)

Catalog No. ABIN503023

This Rabbit Polyclonal antibody specifically detects B3GALTL in WB. It exhibits reactivity toward Human, Mouse, Dog and Rat.

Quick Overview for B3GALTL antibody (Middle Region) (ABIN503023)

Target: B3GALTL (beta 1,3-Galactosyltransferase-Like (B3GALTL))

Reactivity: Human, Mouse, Dog, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B3GALTL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-B3GALTL Antibody

Binding Specificity: Middle Region

Sequence: DYPKDYLSHQ VPISFHKHWN IDPVKVYFTW LAPSDEDKAR QETQKGFREE

Predicted Reactivity: Dog: 93%, Human: 100%, Mouse: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against B3GALTL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human B3GALTL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 498 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for B3GALTL

Target: B3GALTL (beta 1,3-Galactosyltransferase-Like (B3GALTL))

Alternative Name: B3GALTL

Background: B3GALTL is the O-fucosyltransferase that transfers glucose toward fucose with a beta-1,3 linkage. B3GALTL specifically glucosylates O-linked fucosylglycan on TSP type-1 domains of proteins, thereby contributing to elongation of O-fucosylglycan.B3GALTL is a beta-1,3-glucosyltransferase involved in the synthesis of the unusual O-linked disaccharide glucosyl-beta-1,3-fucose-O- found on the thrombospondin (see THBS1, MIM 188060) type-1 repeats (TSRs) of many biologically important proteins. Biosynthesis of glucosyl-beta-1,3-fucose-O- is initiated by protein O-fucosyltransferase-2 (POFUT2, MIM 610249), which attaches the fucosyl residue to a serine or threonine within the TSR. B3GALTL subsequently transfers the glucose onto TSR-fucose (Hess et al., 2008 [PubMed 18199743]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-270 DN999521.1 41-310 271-457 AB101481.1 161-347 458-467 DN999521.1 499-508 468-1217 AB101481.1 358-1107 1218-1279 DA290408.1 515-576 1280-1607 AB101481.1 1170-1497 1608-2495 AY190526.1 1498-2385 2496-3459 AY190526.1 2387-3350 3460-4071 AV728071.1 87-698 4072-4221 AA769548.1 1-150 c
Alias Symbols: B3GTL, B3Glc-T, Gal-T, beta3Glc-T, B3GLCT
Protein Interaction Partner: UBC, ELAVL1,
Protein Size: 498

Molecular Weight: 56 kDa

Gene ID: 145173

NCBI Accession: NM_194318, NP_919299

UniProt: Q6Y288