Sperm Associated Antigen 6 (SPAG6) (Middle Region) antibody

Details for Product No. ABIN503491
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Synonyms Spag6, SPAG6, pf16, repro-sa-1, DKFZp459D035, Repro-SA-1, PF16, RGD1310892, zgc:91805
Middle Region
(3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(33), (17), (17)
(28), (5)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(22), (10), (7), (5), (1)
Pubmed 1 reference available
Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Immunogen Synthetic peptide directed towards the middle region of human SPAG6
Predicted Reactivity African clawed frog : 100 %, Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %, Guinea pig : 90 %
Characteristics This is a rabbit polyclonal antibody against SPAG6. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name SPAG6 (SPAG6 Antibody Abstract)
Background SPAG6 is important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Molecular Weight 56 kDa
Gene ID 9576
NCBI Accession NP_036575, NM_012443
UniProt O75602
Research Area Stem Cells, Cytoskeleton
Application Notes Optimal working dilutions should be determined experimentally by the investigator.

Antigen size: 509 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Sperm Associated Antigen 6 (SPAG6) (Middle Region) antibody (ABIN503491) WB Suggested Anti-SPAG6 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Product cited in: Mehrle, Rosenfelder, Schupp et al.: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, 2005 (PubMed).

Catalog No. ABIN503491
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