SPAG6 antibody (Middle Region)

Catalog No. ABIN503491

Product Details anti-SPAG6 Antibody

Target: SPAG6 (Sperm Associated Antigen 6 (SPAG6))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Cow, Dog, Horse, Zebrafish (Danio rerio), Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG6 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HVVGQFSKVL PHDSKARRLF VTSGGLKKVQ EIKAEPGSLL QEYINSINSC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SPAG6. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SPAG6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SPAG6 antibody (ABIN503491)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for SPAG6

Target: SPAG6 (Sperm Associated Antigen 6 (SPAG6))

Alternative Name: SPAG6 (SPAG6 Products)

Synonyms: Repro-SA-1 antibody, pf16 antibody, PF16 antibody, RGD1310892 antibody, zgc:91805 antibody, SPAG6 antibody, repro-sa-1 antibody, DKFZp459D035 antibody, sperm associated antigen 6 antibody, sperm associated antigen 6-like antibody, sperm associated antigen 6 L homeolog antibody, SPAG6 antibody, Spag6l antibody, Spag6 antibody, spag6 antibody, spag6.L antibody

Background: SPAG6 is important for structural integrity of the central apparatus in the sperm tail and for flagellar motility.The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Alias Symbols: DKFZp434I153, MGC26276, Repro-SA-1, pf16
Protein Interaction Partner: APP, SPAG16,
Protein Size: 509

Molecular Weight: 56 kDa

Gene ID: 9576

NCBI Accession: NM_012443, NP_036575

UniProt: O75602