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Histone H2B Type 2-F (HIST2H2BF) (N-Term) antibody

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Antigen
Epitope
N-Term
(18), (15), (2)
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(20), (1), (1)
Host
Rabbit
(20)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
(18), (14)
Pubmed 1 reference available
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Quantity 50 μg
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Immunogen Synthetic peptide directed towards the N terminal of human HIST2H2BF
Blocking Peptide Blocking peptide for this product available: ABIN978010
Sequence MPDPAKSAPA PKKGSKKAVT KVQKKDGKKR KRSRKESYSV YVYKVLKQVH
Predicted Reactivity Human : 100 %, Rabbit : 100 %
Characteristics This is a rabbit polyclonal antibody against HIST2H2BF. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name HIST2H2BF (HIST2H2BF Antibody Abstract)
Background HIST2H2BF is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Molecular Weight 14 kDa
Gene ID 440689
NCBI Accession NP_001019770, NM_001024599
UniProt Q5QNW6
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 126 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
Western Blotting (WB) image for anti-Histone H2B Type 2-F (HIST2H2BF) (N-Term) antibody (ABIN503884) WB Suggested Anti-HIST2H2BF Antibody Titration: 0.2-1 ug/ml
ELISA Titer:...
Chromatin Immunoprecipitation (ChiP) image for anti-Histone H2B Type 2-F (HIST2H2BF) (N-Term) antibody (ABIN503884) Chromatin Immunoprecipitation (ChIP) Using HIST2H2BF Antibody - N-terminal region (AR...
Background publications Kim, Sprung, Chen et al.: "Substrate and functional diversity of lysine acetylation revealed by a proteomics survey." in: Molecular cell, Vol. 23, Issue 4, pp. 607-18, 2006 (PubMed).

Catalog No. ABIN503884
$289.00
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