Mitochondrial Ribosomal Protein S15 (MRPS15) (Middle Region) antibody

Details for Product No. ABIN504371
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Antigen
Synonyms CG4207, DmMRPS15, Dmel\\CG4207, MRP-S15, mRpS15, zgc:92862, MPR-S15, RPMS15, S15mt, 1500003E24Rik, 2410002B11Rik, Mprs15
Epitope
Middle Region
(8), (7), (4), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Human
(20), (4), (3), (1)
Host
Rabbit
(20)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(20), (9), (6), (4), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN504371
289.00 $
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Immunogen Synthetic peptide directed towards the middle region of human MRPS15
Sequence QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS
Predicted Reactivity Human : 100 %, Rabbit : 80 %, Bovine : 70 %, Guinea pig : 70 %, Mouse : 70 %
Characteristics This is a rabbit polyclonal antibody against MRPS15. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name MRPS15
Background MRPS15 is a 28S subunit protein that belongs to the ribosomal protein S15P family. The protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the protein is the least conserved among small subunit ribosomal proteins.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.
Molecular Weight 30 kDa
Gene ID 64960
NCBI Accession NP_112570, NM_031280
UniProt P82914
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 257 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Mitochondrial Ribosomal Protein S15 (MRPS15) (Middle Region) antibody WB Suggested Anti-MRPS15 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Liver
Background publications Zhang, Gerstein: "Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome." in: Genomics, Vol. 81, Issue 5, pp. 468-80, 2003 (PubMed).

Validation Images
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