Runt-Related Transcription Factor 1; Translocated To, 1 (Cyclin D-Related) (RUNX1T1) antibody

Antigen: Runt-Related Transcription Factor 1; Translocated To, 1 (Cyclin D-Related) (RUNX1T1)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Chicken, Human, Mouse (Murine), Rat (Rattus), Zebrafish, Xenopus laevis

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN504421

Additional Information

Alternative name: RUNX1T1

Gene ID: RUNX1T1

UniProt: B2R6I9

Immunogen: The immunogen for anti-RUNX1T1 antibody: synthetic peptide directed towards the middle region of human RUNX1T1

Sequence: RQCNLQQFIQQTGAALPPPPRPDRGPPGTQGPLPPAREES LLGAPSESHA

Cross-Reactivity: Chicken, Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-RUNX1T1 antibody concentration is 1 mg/ml.

Description: RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.

Characteristics: Antigen Size: 577 amino acids

Molecular Weight: 64kDa

Application Details

Application Notes: WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate

Publications

Product: Salat, Liefke, Wiedenmann et al.: "ETO, but not leukemogenic fusion protein AML1/ETO, augments RBP-Jkappa/SHARP-mediated repression of notch target genes." in: Molecular and cellular biology, Vol. 28, Issue 10, pp. 3502-12, 2008 (PubMed).