RUNX1T1 antibody (Middle Region)

Catalog No. ABIN504421

This Rabbit Polyclonal antibody specifically detects RUNX1T1 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Cow and Pig.

Quick Overview for RUNX1T1 antibody (Middle Region) (ABIN504421)

Target: RUNX1T1 (Runt-Related Transcription Factor 1, Translocated To, 1 (Cyclin D-Related) (RUNX1T1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX1T1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-RUNX1T1 Antibody

Binding Specificity: Middle Region

Sequence: RQCNLQQFIQ QTGAALPPPP RPDRGPPGTQ GPLPPAREES LLGAPSESHA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 86%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against RUNX1T1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RUNX1T1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 577 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RUNX1T1

Target: RUNX1T1 (Runt-Related Transcription Factor 1, Translocated To, 1 (Cyclin D-Related) (RUNX1T1))

Alternative Name: RUNX1T1

Background: RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8,21)(q22,q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.
Alias Symbols: AML1T1, CBFA2T1, CDR, ETO, MGC2796, MTG8, MTG8b, ZMYND2
Protein Interaction Partner: EPS8, NECAB2, ABI3, GSE1, MID2, SPRY2, HOMER3, LPXN, ZMYM4, STX11, MEOX2, CCDC36, TRIM42, SPERT, CREB3L1, LZTS2, EFHC2, CPSF7, C19orf57, PRDM14, CBFA2T2, NCOR1, UBQLN4, ATN1, BCL6, ETS1, RUNX1, DNMT1, SPEN, RBPJ, UBE2E2, UBC, TRIM33, HDAC1, NCOR2, SIN3A,
Protein Size: 577

Molecular Weight: 64 kDa

Gene ID: 862

NCBI Accession: NM_004349, NP_004340

UniProt: B2R6I9