2B4 antibody (Internal Region, pTyr271)

Catalog No. ABIN487961

Product Details anti-2B4 Antibody

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Binding Specificity: Internal Region, pTyr271

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This 2B4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: c-EFLTIPYEDV KD

Specificity: This antibody recognizes CD244.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_057466.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western blot: Approx 75 kDa band observed in Human Natural Killer (NK) cell lysates afterimmunoprecipitation with mouse monoclonal antibody C1.7 coupled to Protein G agarose(calculated MW of 41.5 kDa according to NP_057466.1). We do note that CD244 is aglycoprotein which makes a higher size band to be expected. Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for 2B4

Target: 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))

Alternative Name: CD244 (CD244 Products)

Synonyms: CD244 antibody, 2B4 antibody, NAIL antibody, NKR2B4 antibody, Nmrk antibody, SLAMF4 antibody, C9.1 antibody, F730046O15Rik antibody, Ly90 antibody, CD244 molecule antibody, CD244 natural killer cell receptor 2B4 antibody, CD244 antibody, Cd244 antibody

Background: CD244 is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. CD244 activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Although there have been several molecules shown to interact with CD244, the signaling mechanism ofCD244-mediated activation of NK cells is still unknown. It is a high affinity receptor for CD48.Synonyms: NAIL, NK cell activation-inducing ligand, NK cell type I receptor protein 2B4, NKR2B4, Natural killer cell receptor 2B4

Gene ID: 51744

NCBI Accession: NP_057466

UniProt: Q9BZW8