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NOTUM antibody (N-Term)

NOTUM Reactivity: Human, Mouse, Rat WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN500375
  • Target See all NOTUM Antibodies
    NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))
    Binding Specificity
    • 15
    • 8
    • 7
    • 6
    • 6
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    Human, Mouse, Rat
    Host
    • 37
    Rabbit
    Clonality
    • 37
    Polyclonal
    Conjugate
    • 9
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NOTUM antibody is un-conjugated
    Application
    • 33
    • 21
    • 13
    • 13
    • 4
    • 3
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects Notum.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Mouse, Rat.
    Purification
    Peptide Affinity Chromatography
    Immunogen
    NOTUM antibody was raised against a 19 amino acid peptide near the amino terminus of Human NOTUM.
    Isotype
    IgG
    Top Product
    Discover our top product NOTUM Primary Antibody
  • Application Notes
    ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS containing 0.02 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))
    Alternative Name
    Notum (NOTUM Products)
    Synonyms
    5730593N15Rik antibody, RGD1307119 antibody, notum, palmitoleoyl-protein carboxylesterase antibody, notum pectinacetylesterase homolog (Drosophila) antibody, NOTUM, palmitoleoyl-protein carboxylesterase antibody, NOTUM antibody, Notum antibody
    Background
    In Drosophila, the notum gene is regulated by the Wingless pathway and encodes a secreted hydrolase that modifies heparan sulfate proteoglycans. The mammalian homolog has been shown to be able to cleave glypicans and can release GPI-anchored proteins from the mammalian cell surface. Like the Drosophilia NOTUM, the mammalian protein can act as a negative regulator of the Wnt signaling pathway. NOTUM is expressed at a low level in most mammalian tissues, although it is overexpressed in a subset of human hepatocellular carcinomas. Its transcription is regulated by b-catenin/TCF and is a target of the Wnt signaling pathway, forming a negative feedback loop that regulates the expression and activity of the Wnt pathway.
    Gene ID
    147111
    NCBI Accession
    NP_848588
    UniProt
    Q6P988
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