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GST-Tag antibody

Antigen

GST-Tag
Clonality Monoclonal (GST-B6)
Host
Alternatives

Mouse
Reactivity
Conjugate
Alternatives Un-conjugated
Application
Alternatives Dot Blot (Dot), ELISA, Immunoprecipitation (IP), Immunostaining (ISt), Western Blotting (WB)
4 references available
Catalog no. ABIN559685
Quantity 100 µg  (1mg/mL)
Price 196.90 $   Plus shipping costs $45.00
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anti-GST-Tag antibody

Additional Information
Application Details Publications Images Alternatives back to top

Alternative name GST Tag
Immunogen GST protein
Isotype IgG2a
Clone GST-B6
Specificity Recognizes native and denatured forms of purified GST or GST fusion proteins
Sensitivity WB (with ECL): 10-50ng of purified GST or GST fusion proteins

Application Details
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Application Notes WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Concentration 1mg/mL
Purification Protein A affinity chromatography from mouse ascites fluid
Buffer 10mM PBS, pH 7.2, 0.05% NaN3
Storage Store at -20°C for up to 2 years or more. Centrifuge after first thaw to maximize product recovery. Avoid repeated freeze-thaw cycles by aliquots. Store aliquots at 4°C for several days to weeks
Restrictions For Research Use only

Publications
Application Details Images Alternatives back to top

Product OConnor, Strasser, OReilly et al.: "Bim: a novel member of the Bcl-2 family that promotes apoptosis." in: The EMBO journal, Vol. 17, Issue 2, pp. 384-95, 1998 (PubMed).

Hsu, Lin, Hsueh: "BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members." in: Molecular endocrinology (Baltimore, Md.), Vol. 12, Issue 9, pp. 1432-40, 1998 (PubMed).

Adams, Cory: "The Bcl-2 protein family: arbiters of cell survival." in: Science (New York, N.Y.), Vol. 281, Issue 5381, pp. 1322-6, 1998 (PubMed).

Gross, McDonnell, Korsmeyer: "BCL-2 family members and the mitochondria in apoptosis." in: Genes & development, Vol. 13, Issue 15, pp. 1899-911, 1999 (PubMed).