RFP antibody (N-Term)

Catalog No. ABIN559690

This Mouse Monoclonal antibody specifically detects RFP in WB, ELISA, IF, IHC, IP and DB. It exhibits reactivity toward Discosoma.

Quick Overview for RFP antibody (N-Term) (ABIN559690)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Dot Blot (DB)

Clone: RF5R

Product Details anti-RFP Antibody

Binding Specificity: N-Term

Specificity: Recognizes native and denatured forms of RFP and its variants: tag-RFP, turbo-RFP, DeRed, mCherry, mOrange, etc

Purification: Protein A affinity chromatography from mouse ascites fluid

Immunogen: RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates

Isotype: IgG1

Application Details

Application Notes: WB (with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature) / IS: 1:500-2000 dilution / For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Comment:

WB (with ECL): 1-10ng of purified RFP or RFP fusion proteins

Restrictions: For Research Use only

Handling

Concentration: 1mg/mL

Buffer: 10mM PBS, pH 7.2, 0.05% NaN3

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP