IRF1 antibody (AA 216-325)

Catalog No. ABIN561520

This Mouse Monoclonal antibody specifically detects IRF1 in WB and ELISA. It exhibits reactivity toward Human and has been mentioned in 7+ publications.

Quick Overview for IRF1 antibody (AA 216-325) (ABIN561520)

Target: IRF1 (Interferon Regulatory Factor 1 (IRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2E4

Product Details anti-IRF1 Antibody

Binding Specificity: AA 216-325

Purpose: Mouse monoclonal antibody raised against a partial recombinant IRF1.

Sequence: PMPSTSEATT DEDEEGKLPE DIMKLLEQSE WQPTNVDGKG YLLNEPGVQP TSVYGDFSCK EEPEIDSPGG DIGLSLQRVF TDLKNMDATW LDSLLTPVRL PSIQAIPCAP

Cross-Reactivity: Human

Characteristics: Antibody Reactive Against Recombinant Protein.

Immunogen: IRF1 (NP_002189, 216 a.a. ~ 325 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: In 1x PBS, pH 7.4

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References for anti-IRF1 antibody (ABIN561520)

Bartlett, Slater, Glanville, Haas, Caramori, Casolari, Clarke, Message, Aniscenko, Kebadze, Zhu, Mallia, Mizgerd, Belvisi, Papi, Kotenko, Johnston, Edwards: "Defining critical roles for NF-?B p65 and type I interferon in innate immunity to rhinovirus." in: EMBO molecular medicine, Vol. 4, Issue 12, pp. 1244-60, (2012) (PubMed).

Baumjohann, Okada, Ansel: "Cutting Edge: Distinct waves of BCL6 expression during T follicular helper cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 5, pp. 2089-92, (2011) (PubMed).

Choi, Kageyama, Eto, Escobar, Johnston, Monticelli, Lao, Crotty: "ICOS receptor instructs T follicular helper cell versus effector cell differentiation via induction of the transcriptional repressor Bcl6." in: Immunity, Vol. 34, Issue 6, pp. 932-46, (2011) (PubMed).

Fazilleau, McHeyzer-Williams, Rosen, McHeyzer-Williams: "The function of follicular helper T cells is regulated by the strength of T cell antigen receptor binding." in: Nature immunology, Vol. 10, Issue 4, pp. 375-84, (2009) (PubMed).

Nurieva, Chung, Martinez, Yang, Tanaka, Matskevitch, Wang, Dong: "Bcl6 mediates the development of T follicular helper cells." in: Science (New York, N.Y.), Vol. 325, Issue 5943, pp. 1001-5, (2009) (PubMed).

Steffen, MacKay, Kerr, Tsuchiya, Hu, Fox, Dang, Johnston, Oakes, Tchao, Pak, Fields, Kennedy, Kaeberlein: "Yeast life span extension by depletion of 60s ribosomal subunits is mediated by Gcn4." in: Cell, Vol. 133, Issue 2, pp. 292-302, (2008) (PubMed).

Ye, Lista, Lo Coco, Knowles, Offit, Chaganti, Dalla-Favera: "Alterations of a zinc finger-encoding gene, BCL-6, in diffuse large-cell lymphoma." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 747-50, (1993) (PubMed).

Target Details for IRF1

Target: IRF1 (Interferon Regulatory Factor 1 (IRF1))

Alternative Name: IRF1

Background: Full Gene Name: interferon regulatory factor 1
Synonyms: IRF-1,MAR

Gene ID: 3659

NCBI Accession: NM_002198

Pathways: Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Immune Effector Process, Hepatitis C, Autophagy