TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody

Details for Product No. ABIN565080
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Antigen
Synonyms TARDBP, TDP43, DKFZp459I2127, tardbp, ALS10, TDP-43, 1190002A23Rik, C85084, Tdp43, wu:fb77f02, wu:fc52g10
Epitope
AA 1-260
(28), (24), (15), (14), (14), (9), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(149), (54), (45), (11), (8), (7), (6), (5), (4), (2), (1), (1)
Host
Mouse
(106), (34), (14), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), ELISA, Immunoprecipitation (IP), RNA Interference (RNAi)
(137), (58), (49), (36), (26), (17), (10), (9), (5), (4), (4), (3), (2), (1), (1), (1)
Pubmed 21 references available
Quantity 100 μg
Shipping to United States (Change)
Availability Will be delivered in 11 to 12 Business Days
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Catalog No. ABIN565080
330.00 $
Plus shipping costs $45.00 and $22.00 dry ice

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Immunogen TARDBP (AAH01487.1, 1 a.a. ~ 260 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen sequence: MSEYIRVTEDENDEPIEIPS EDDGTVLLSTVTAQFPGACG LRYRNPVSQCMRGVRLVEGI LHAPDAGWGNLVYVVNYPKD NKRKMDETDASSAVKVKRAV QKTSDLIVLGLPWKTTEQDL KEYFSTFGEVLMVQVKKDLK TGHSKGFGFVRFTEYETQVK VMSQRHMIDGRWCDCKLPNS KQSQDEPLRSRKVFVGRCTE DMTEDELREFFSQYGDVMDV FIPKPFRAFAFVTFADDQIA QSLCGEDLIIKGISVHISNA
Clone 2E2-D3
Isotype IgG1 kappa
Characteristics Mouse monoclonal antibody raised against a full length recombinant TARDBP.
Alternative Name TARDBP
Background Gene: TAR DNA binding protein
Synonyms: ALS10, TDP-43
Gene ID 23435
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Application Notes Application specialization: Cell lysate, Recombinant protein, Transfected lysate
Restrictions For Research Use only
Buffer 1x PBS, pH 7.2
Storage -20 °C
Storage Comment Store at -20 °C or lower. Aliquot to avoid repeated freezing and thawing.
Supplier Images
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody Immunoperoxidase of monoclonal antibody to TARDBP on formalin-fixed paraffin-embedded human leiomyosarcoma tissue [antibody concentration 3 µg/mL]
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (2) Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell. [antibody concentration 10 µg/mL]
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (3) TARDBP monoclonal antibody (M01), clone 2E2-D3 Western Blot analysis of TARDBP expression in A-431 (ABIN1339532).
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (4) Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody (M01), clone 2E2-D3 (ABIN565080). GAPDH (36.1 kDa) used as specificity and loading control.
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (5) Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody (M01), clone 2E2-D3.

Lane 1: TARDBP transfected lysate(44.7 kDa).
Lane 2: Non-transfected lysate.
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (6) Immunoprecipitation of TARDBP transfected lysate using anti-TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP MaxPab rabbit polyclonal antibody.
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (7) Detection limit for recombinant GST tagged TARDBP is 0.3 ng/mL as a capture antibody.
anti-TAR DNA Binding Protein (TARDBP) (AA 1-260) antibody (8) Western Blot detection against Immunogen (54.34 KDa) .
Product cited in: Ohta, Tremblay, Schneider et al.: "Interaction of transactive response DNA binding protein 43 with nuclear factor κB in mild cognitive impairment with episodic memory deficits." in: Acta neuropathologica communications, Vol. 2, Issue 1, pp. 37, 2014 (PubMed).

Cortese, Plagnol, Brady et al.: "Widespread RNA metabolism impairment in sporadic inclusion body myositis TDP43-proteinopathy." in: Neurobiology of aging, Vol. 35, Issue 6, pp. 1491-8, 2014 (PubMed).

De Marco, Lomartire, Mandili et al.: "Reduced cellular Ca(2+) availability enhances TDP-43 cleavage by apoptotic caspases." in: Biochimica et biophysica acta, Vol. 1843, Issue 4, pp. 725-34, 2014 (PubMed).

Yan, Wang, Wei et al.: "TDP-43 causes differential pathology in neuronal versus glial cells in the mouse brain." in: Human molecular genetics, Vol. 23, Issue 10, pp. 2678-93, 2014 (PubMed).

Suárez-Calvet, Dols-Icardo, Lladó et al.: "Plasma phosphorylated TDP-43 levels are elevated in patients with frontotemporal dementia carrying a C9orf72 repeat expansion or a GRN mutation." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 85, Issue 6, pp. 684-91, 2014 (PubMed).

Hebron, Chen, Miessau et al.: "Parkin reverses TDP-43-induced cell death and failure of amino acid homeostasis." in: Journal of neurochemistry, Vol. 129, Issue 2, pp. 350-61, 2014 (PubMed).

Iovino, Pfisterer, Holton et al.: "The novel MAPT mutation K298E: mechanisms of mutant tau toxicity, brain pathology and tau expression in induced fibroblast-derived neurons." in: Acta neuropathologica, Vol. 127, Issue 2, pp. 283-95, 2014 (PubMed).

Grau-Rivera, Gelpi, Rey et al.: "Prominent psychiatric symptoms in patients with Parkinson's disease and concomitant argyrophilic grain disease." in: Journal of neurology, Vol. 260, Issue 12, pp. 3002-9, 2013 (PubMed).

Finsterer, Stöllberger, Kovacs: "Asymptomatic hyper-creatine-kinase-emia as sole manifestation of inclusion body myositis." in: Neurology international, Vol. 5, Issue 2, pp. 34-6, 2013 (PubMed).

Nishimoto, Nakagawa, Hirose et al.: "The long non-coding RNA nuclear-enriched abundant transcript 1_2 induces paraspeckle formation in the motor neuron during the early phase of amyotrophic lateral sclerosis." in: Molecular brain, Vol. 6, pp. 31, 2013 (PubMed).

Mundiñano, Hernandez, Dicaudo et al.: "Reduced cholinergic olfactory centrifugal inputs in patients with neurodegenerative disorders and MPTP-treated monkeys." in: Acta neuropathologica, Vol. 126, Issue 3, pp. 411-25, 2013 (PubMed).

Tong, Huang, Bi et al.: "Expression of ALS-linked TDP-43 mutant in astrocytes causes non-cell-autonomous motor neuron death in rats." in: The EMBO journal, Vol. 32, Issue 13, pp. 1917-26, 2013 (PubMed).

Gelpi, Cullel, Navarro-Otano et al.: "Globular glial-like inclusions in a patient with advanced Alzheimer's disease." in: Acta neuropathologica, Vol. 126, Issue 1, pp. 155-7, 2013 (PubMed).

Dayton, Gitcho, Orchard et al.: "Selective forelimb impairment in rats expressing a pathological TDP-43 25 kDa C-terminal fragment to mimic amyotrophic lateral sclerosis." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 21, Issue 7, pp. 1324-34, 2013 (PubMed).

Iyer, Prabowo, Anink et al.: "Cell injury and premature neurodegeneration in focal malformations of cortical development." in: Brain pathology (Zurich, Switzerland), Vol. 24, Issue 1, pp. 1-17, 2013 (PubMed).

Iranzo, Tolosa, Gelpi et al.: "Neurodegenerative disease status and post-mortem pathology in idiopathic rapid-eye-movement sleep behaviour disorder: an observational cohort study." in: Lancet neurology, Vol. 12, Issue 5, pp. 443-53, 2013 (PubMed).

Bi, Huang, Tong et al.: "Reactive astrocytes secrete lcn2 to promote neuron death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 10, pp. 4069-74, 2013 (PubMed).

Doherty, Rohrer, Lees et al.: "Primary progressive aphasia with parkinsonism." in: Movement disorders : official journal of the Movement Disorder Society, Vol. 28, Issue 6, pp. 741-6, 2013 (PubMed).

Hebron, Lonskaya, Sharpe et al.: "Parkin ubiquitinates Tar-DNA binding protein-43 (TDP-43) and promotes its cytosolic accumulation via interaction with histone deacetylase 6 (HDAC6)." in: The Journal of biological chemistry, Vol. 288, Issue 6, pp. 4103-15, 2013 (PubMed).

Swarup, Audet, Phaneuf et al.: "Abnormal regenerative responses and impaired axonal outgrowth after nerve crush in TDP-43 transgenic mouse models of amyotrophic lateral sclerosis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 32, Issue 50, pp. 18186-95, 2012 (PubMed).

Watanabe, Kaneko, Yamanaka: "Accelerated disease onset with stabilized familial amyotrophic lateral sclerosis (ALS)-linked mutant TDP-43 proteins." in: The Journal of biological chemistry, Vol. 288, Issue 5, pp. 3641-54, 2013 (PubMed).

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