MIF antibody (Middle Region)

Catalog No. ABIN567342

The Rabbit Polyclonal anti-MIF antibody (ABIN567342) specifically detects MIF in WB and IHC. The antibody is reactive with Human, Mouse, Rat, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse and Zebrafish (Danio rerio) samples.

Quick Overview for MIF antibody (Middle Region) (ABIN567342)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human, Mouse, Rat, Cow, Sheep, Rabbit, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MIF Antibody

Binding Specificity: Middle Region

Sequence: PQYIAVHVVP DQLMAFGGSS EPCALCSLHS IGKIGGAQNR SYSKLLCGLL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against MIF. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MIF

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 115 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways.
Alias Symbols: GIF, GLIF, MMIF
Protein Interaction Partner: WDYHV1, UBC, MIF, MDM2, ASB15, ZNF408, UPF2, BNIPL, HCVgp1, NGFRAP1, VCAM1, ITGA4, FN1, IGBP1, CLNS1A, COPS5, TP53, NME1, CDK2, STUB1, HSP90AA1, VHL, STRN4, COPS6, GORASP2, CD74, FIBP,
Protein Size: 115

Molecular Weight: 12 kDa

Gene ID: 4282

NCBI Accession: NM_002415, NP_002406

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling