POLD1 antibody

Catalog No. ABIN567776

The Rat Monoclonal anti-POLD1 antibody is suitable to detect POLD1 in samples from Human, Rat and Mouse. It has been validated for WB.

Quick Overview for POLD1 antibody (ABIN567776)

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Reactivity: Human, Rat, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This POLD1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5G1

Product Details anti-POLD1 Antibody

Specificity: This antibody reacts with DNA polymerase delta p125 catalytic subunit (125 kDa) by Western blotting.

Purification: Protein-G Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant full length His-Human p125.

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Alternative Name: POLD1

Background: In eukaryotic cells, 3 types of DNA polymerases (pol), pol alpha, pol delta and pol epsilon function in accurate and efficient DNA replication. Pol delta is a heteromultimeric enzyme composed of a 125 kDa major catalytic subunit and three smaller accessory subunits. Pol delta requires a number of accessory molecules to exert its full activity. Proliferating cell nuclear antigen (PCNA) forms a doughnut-shaped trimer with a 35 Ä hole and works as the sliding clamp and replication factor C acts as the clamp loader to improve Pol delta processivity. Pol delta is also involved in nucleotide excision repair of DNA.Synonyms: DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125

Gene ID: 5424

NCBI Accession: NP_002682

UniProt: P28340

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Chromatin Binding, Synthesis of DNA