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OCT4 antibody (Internal Region)
POU5F1
Reactivity: Mouse, Rat
WB, EIA
Host: Goat
Polyclonal
unconjugated
Product Details anti-OCT4 Antibody
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Target
See all OCT4 (POU5F1) Antibodies
OCT4 (POU5F1)
(POU Class 5 Homeobox 1 (POU5F1))
Binding Specificity
All epitopes for OCT4 antibodies
Internal Region
Reactivity
All reactivities for OCT4 antibodies
Mouse, Rat
Host
All hosts for OCT4 antibodies
Goat
Clonality
All clonalities for OCT4 antibodies
Polyclonal
Conjugate
All conjugates for OCT4 antibodies
This OCT4 antibody is un-conjugated
Application
All applications for OCT4 antibodies
Western Blotting (WB), Enzyme Immunoassay (EIA)
Sequence
C-DRPNAVKLEK VEP
Specificity
Recognizes OCT4/POU5F1.
Cross-Reactivity (Details)
Species reactivity (expected):Rat. Species reactivity (tested):Mouse.
Purification
Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
Immunogen
Peptide with sequence from the internal region of the protein sequence according to NP_038661.2.
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Alternatives
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Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target Details for OCT4
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Target
OCT4 (POU5F1)
(POU Class 5 Homeobox 1 (POU5F1))
Alternative Name
OCT3/4 (POU5F1 Products )
Synonyms
OCT3 antibody, OCT4 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, Oct-3 antibody, Oct-4 antibody, POU5F1 antibody, gp9 antibody, gp-9 antibody, oct4 antibody, pou2 antibody, POU-2 antibody, cb197 antibody, spg/pou2 antibody, chunp6868 antibody, wu:fd18d06 antibody, NF-A3 antibody, Oct-3/4 antibody, Oct3 antibody, Oct3/4 antibody, Oct4 antibody, Otf-3 antibody, Otf-4 antibody, Otf3 antibody, Otf3-rs7 antibody, Otf3g antibody, Otf4 antibody, POU class 5 homeobox 1 antibody, POU domain, class 5, transcription factor 3 antibody, POU domain, class 5, transcription factor 1 antibody, POU5F1 antibody, pou5f3 antibody, Pou5f1 antibody
Background
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. The POU-specific domain mediates interaction with PKM2 and is Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.Synonyms: OCT3, OCT4, OTF3, Oct-3, Oct-4, Octamer-binding transcription factor 3, POU domain, POU5F1, class 5, transcription factor 1
Gene ID
18999
NCBI Accession
NP_038661
UniProt
P20263
Pathways
Stem Cell Maintenance
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