ITIH4 antibody (Internal Region)

Catalog No. ABIN570706

Product Details anti-ITIH4 Antibody

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ITIH4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Purpose: ITIH4

Sequence: ERLCVDPRHR QGPVN

Specificity: The immunizing peptide represents a region of the 35 kDa chain. This chain is glycosylated giving rise to an apparent higher molecular weight.

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence ERLCVDPRHRQGPVN, from the internal region of the protein sequence according to NP_002209.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6 μg/mL.

Western Blot: Preliminary experiments gave an approx. 150 kDa band in Human Liver and Lung lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ITIH4

Target: ITIH4 (Inter alpha Globulin Inhibitor H4 (ITIH4))

Alternative Name: ITIH4 (ITIH4 Products)

Synonyms: ITI-HC4 antibody, Itih-4 antibody, GP120 antibody, H4P antibody, IHRP antibody, ITIHL1 antibody, PK-120 antibody, PK120 antibody, inter alpha-trypsin inhibitor, heavy chain 4 antibody, inter-alpha-trypsin inhibitor heavy chain family member 4 antibody, inter-alpha-trypsin inhibitor heavy chain family, member 4 antibody, Itih4 antibody, ITIH4 antibody

Background: ITIH4, inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein), DKFZp686G21125, H4P, IHRP, ITIHL1, PK120, inter-alpha (globulin) inhibitor H4, plasma kallikrein-sensitive glycoprotein

Gene ID: 3700

NCBI Accession: NP_002209

Pathways: Glycosaminoglycan Metabolic Process