IL1R2 antibody (Internal Region)

Catalog No. ABIN570867

The Goat Polyclonal anti-IL1R2 antibody (ABIN570867) specifically detects IL1R2 in WB, ELISA, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for IL1R2 antibody (Internal Region) (ABIN570867)

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IL1R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-IL1R2 Antibody

Binding Specificity: Internal Region

Purpose: IL1R2

Sequence: QYNITRSIEL RIKK

Specificity: This antibody is expected to cross-react with both reported isoforms (NP_004624.1, NP_001248348.1). Reported variants represent identical protein: NP_775465.1, NP_004624.1

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QYNITRSIELRIKK

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 45 kDa band observed in lysates of cell lines LNCaP and MOLT-4 (calculated MW o 45.4 kDa according to NP_004624.1). Recommended concentration:0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentrat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IL1R2

Target: IL1R2 (Interleukin 1 Receptor, Type II (IL1R2))

Alternative Name: IL1R2

Background: IL1R2, interleukin 1 receptor, type II, CD121b, IL1RB, MGC47725, antigen CDw121b, type II interleukin-1 receptor, beta

Gene ID: 7850

NCBI Accession: NP_004624, NP_001248348

Pathways: NF-kappaB Signaling